FM3Ats C1.T85

Tumourigenicity studies, chromosome condensation studies.

A temperature sensitive mutant isolated from the mouse mammary cell carcinoma cell line, FM3A (Sigma Catalogue number. 87100804). Cells will not divide at 39°C (non-permissive temperature) and will grow at 33°C (permissive temperature). Electron microscope findings have shown that chromatin abnormally condenses into fragmented and compact forms in 80% of the cells at 39°C for 16 hours. This abnormal condensation does not occur at 33°C or in the FM3A cell line at either temperature.

Mouse C3H mammary carcinoma

Maintain cultures between 1-3x100,000 cells/ml; 5% CO₂; 33°C.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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