生物来源
human lung
包装
tube of 5 μg 87112508-DNA-5UG
pkg of vial of cells 87112508-1VL
生长模式
Adherent
核型
2n = 46; diploid
形态学
Fibroblast-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies
生化/生理作用
Derived from a biopsy taken from a 26 year old Caucasian male with idiopathic pulmonary fibrosis.
Human Caucasian diploid lung
制备说明
Coon′s modified Ham′s F12 or Kaign′s modified Ham′s F12 + 45mg/L ascorbic acid + 18mg/L Inositol + 2mM Glutamine + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. A further 9 population doublings expected.
其他说明
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法规信息
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