biological source
mouse breast
packaging
tube of 5 μg 87121104-DNA-5UG, pkg of vial of cells 87121104-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Mastocytoma
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Genetic, transfection and tumourigenicity studies
Biochem/physiol Actions
From P815 after serial cycles of transfection. This HTR variant shows high efficiency of transfection (0.00001) for neomycin-resistance and TK gene or other exogenous DNA. Possible to select TK- cells with BUdR for resubmission to transfection with TK gene.
Mouse DBA/2 mastocytoma
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 8% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.