biological source
mouse breast
packaging
tube of 5 μg 87121104-DNA-5UG, pkg of vial of cells 87121104-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Mastocytoma
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Genetic, transfection and tumourigenicity studies
Biochem/physiol Actions
From P815 after serial cycles of transfection. This HTR variant shows high efficiency of transfection (0.00001) for neomycin-resistance and TK gene or other exogenous DNA. Possible to select TK- cells with BUdR for resubmission to transfection with TK gene.
Mouse DBA/2 mastocytoma
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 8% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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