生物来源
human blood (leukemic T-cell lymphoblast)
包装
tube of 5 μg 88042803-DNA-5UG
pkg of vial of cells 88042803-1VL
生长模式
Suspension
核型
Pseudodiploid, modal No 46
形态学
Not specified
产品
Interleukin 2 (IL-2), human alpha interferon
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Human leukaemic T cell lymphoblast
细胞系描述
Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
应用
IL-2 assay and cloning studies
Jurkat E6.1 cell line has been used to:
- study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
- determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
- study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro
DNA图谱分析
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
培养基
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
传代培养常规
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.
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