biological source
human blood (leukemic T-cell lymphoblast)
packaging
tube of 5 μg 88042803-DNA-5UG, pkg of vial of cells 88042803-1VL
growth mode
Suspension
karyotype
Pseudodiploid, modal No 46
morphology
Not specified
products
Interleukin 2 (IL-2), human alpha interferon
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Jurkat E6.1 cell line has been used to:
- study cell-independent/vector-free delivery of bovine serum albumin-fluorescein conjugate
- determine the cytotoxicity of the cytolethal distending toxin (CDT) holotoxin and its components
- study the expression of bisphenol A exposed estrogen receptor-β (ERβ) and estrogen-related-receptor-α (ERRα) in vitro
IL-2 assay and cloning studies
Biochem/physiol Actions
Derived from Jurkat FHCRC. An IL-2 producing cell line, derived by incubating the cells at 41°C for 48 hours followed by a limiting dilution cloning over macrophages.
Human leukaemic T cell lymphoblast
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
CSF1PO: 11,12
D13S317: 8,12
D16S539: 11
D5S818: 9
D7S820: 8,10
THO1: 6,9.3
TPOX: 8,10
vWA: 18
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. On resuscitation single cells can be observed, during culture most cells will grow as aggregates.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
法规信息
常规特殊物品
低风险生物材料
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实验方案
Learn how to perform cell migration assays in vitro using Millicell® hanging cell culture inserts and the suspension T-cell lines Jurkat and primary CD4+ cells. Monitor migration by flow cytometry and EZ-MTT assays.
商品
Application note on how the CellASIC® ONIX2 microfluidic system can be used to analyze caspase-3 mediated apoptosis/cell death and cellular hypoxia in live immune and cancer cell lines.