biological source
mouse unknown/unspecified
packaging
tube of 5 μg 88042804-DNA-5UG, pkg of vial of cells 88042804-1VL
growth mode
Adherent
karyotype
Hyperdiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
A9 tumour induced C3H mouse
This cell line was selected by passaging A9 (Sigma Catalogue number. 85011426) through syngenic C3H or semi allogenic mice over a period of three months and uptake was considerably higher than A9, thus A9 high uptake or A9HT. Karyotype is similar to A9 but with a slightly reduced total number of chromosomes, with the modal number of 53. This line lacks inosinic acid pyrophosphorylase.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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