biological source
mink lung
packaging
tube of 5 μg 88050503-DNA-5UG, pkg of vial of cells 88050503-1VL
growth mode
Adherent
karyotype
2n = 30; diploid and pseudodiploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus focus forming assays: MSV, feline sarcoma virus
Biochem/physiol Actions
Derived from trypsinised lungs of several foetuses of Aleutian mink. Used for focus forming assays for murine and feline sarcoma viruses.
Mink lung
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.