biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 88070801-DNA-5UG, pkg of vial of cells 88070801-1VL
ImmunoDon
PVG rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-κ-Chain, mouse
technique(s)
cell culture | mammalian: suitable
isotype
IgG1
shipped in
dry ice
storage temp.
−196°C
Immunogen
Mouse F(antibody′)2
Biochem/physiol Actions
Rat Y3Ag1.2.3 x Rat PVG spleen
The antibody is directed against an epitope on the murine kappa chain.
Preparation Note
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.