biological source
human lymph
packaging
tube of 5 μg 88071302-DNA-5UG, pkg of vial of cells 88071302-1VL
growth mode
Suspension
karyotype
2n = 46, diploid
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Co-cultivation and virus studies: Herpesvirus
Biochem/physiol Actions
Derived from the ascitic fluid of a male with Burkitt′s lymphoma of the liver. The cells contain an unidentified herpes-like virus and have been used in co-cultivation studies to establish lymphocytes in cultures. The cell line is also EBNA positive. Ethnicity: Black.
Human Burkitt′s lymphoma
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
CSF1PO: 10,11
D13S317: 12
D16S539: 10,11
D5S818: 12
D7S820: 8,10
THO1: 7,9
TPOX: 6,8
vWA: 15,19
Preparation Note
Maintain cultures between 4-6x100,000 cells/ml. Add fresh medium every 2-3 days. Maximum cell density is achieved at 2-3x1,000,000 cells/ml.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.