THP 1 Cell Line human

THP 1 cell line human has been used to study:

• bactericidal activity of moxifloxacin against Staphylococcus aureus
• the effect of lipopolysaccharide-mediated inflammatory response in murine versus human cell lines
• the effects of purine nucleosides on the in vitro growth of Cryptosporidium parvum
• the change in gene expression after infection with Mycobacterium tuberculosis
• the induction of cytokines in the cell line in presence of antigens obtained from Clostridium difficile

Derived from the peripheral blood of a 1 year old male with acute monocytic leukaemia. THP-1 cells have Fc and C3b receptors and lack surface and cytoplasmic immunoglobulins. These cells also stain positive for α-napthhyl butyrate esterase, produce lysozymes and are phagocytic (both latex beads and sensitised erythrocytes). THP-1 cells can also restore the response of purified T lymphocytes to Concanavlin A, show increased CO₂ production on phagocytosis and can be differentiated into macrophage-like cells using for example DMSO.

Human monocytic leukaemia

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,13
D13S317: 13
D16S539: 11,12
D5S818: 11,12
D7S820: 10
THO1: 8,9.3
TPOX: 8,11
vWA: 16

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS / FCS).

If starting from a frozen ampoule the cryoprotectant should be removed. Add thawed cells to a conical based centrifuge tube, e.g. 15ml tube, slowly add 4 ml of culture medium to the tube. Take a sample of the cell suspension, e.g. 100μl, to count cells. Centrifuge the cell suspension at low speed i.e. 100 - 150 x g for a maximum of 5 minutes. Remove medium and resuspend the cell pellet at a density of 3 - 5 x 100,000 cells/ml in fresh medium containing 20% serum. Incubate flask at 37°C; 5 - 7% CO2. Check daily. Keep flask in a vertical position until the cells reach the exponential phase of growth. This can take up to 7 days. Once the culture is established the serum concentration can be reduced to 10%. To keep the cells in exponential growth, maintain cultures between 3-8x100,000 cells/ml. Requires 5% DMSO and 95% fetal bovine serum (FBS) as cryoprotectant. Growing orders are recommended due to difficulties that can be experienced during the initial start-up of this cell line. Replacements will be charged at full cost where claims cannot be substantiated.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.