biological source
fish colon (posterior)
packaging
tube of 5 μg 88102401-DNA-5UG, pkg of vial of cells 88102401-1VL
growth mode
Adherent
karyotype
Modal no. 51
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus studies: Inf. pacr. necrotic virus, cat fish tumour, and Granoff′s frog viruses
Biochem/physiol Actions
Derived from tissue posterior to the anus of normal adult minnows. Reported to be capable of growth over a wide temperature range from 4°C-36°C with maximum growth at 34°C. Support growth of the virus of infectious pancreatic necrosis (IPN), cat fish tumour virus (CTV) and Granoff frog virus (FV1). ECHO-II will multiply in FHM but the cytopathic effect is not extensive.
Fish Fat Head minnow
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) +10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3; 34°C. Dislodge cells by agitation and scraping. Cells are reported to multiply over a wide range of temperatures 0°-36°C, with maximum growth at about 34°C. It is recommended that cultures are grown at 34
Other Notes
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法规信息
低风险生物材料
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