WISH (HeLa derivative)
NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
生物来源
human cervix
包装
tube of 5 μg 88102403-DNA-5UG
pkg of vial of cells 88102403-1VL
生长模式
Adherent
核型
2n = 46
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
应用
Virus studies: VSV (Indiana), Ad 3, Poliovirus, measles virus
生化/生理作用
Human cervix carcinoma
Originally derived from human amnion tissue. Have been used in virus studies; susceptible to VSV (Indiana), adenovirus 3 and poliovirus. Useful in differentiating virulent and avirulent measles virus. HeLa marker chromosomes and type A G6PD have been detected. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line must be considered as derived from HeLa. Ethnicity: Black.
STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18
CSF1PO: 9,10
D13S317: 14
D16S539: 9,10
D5S818: 11,12
D7S820: 12
THO1: 7
TPOX: 8,12
vWA: 16,18
制备说明
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:10 i.e. seeding at 1-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
其他说明
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
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