biological source
rat muscle
packaging
tube of 5 μg 88120601-DNA-5UG, pkg of vial of cells 88120601-1VL
growth mode
Adherent
karyotype
2n = 42
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Plaque assay and virus studies: Rous sarcoma virus, MuLV
Biochem/physiol Actions
Original tumour induced in outbred Wistar rats by intramuscular injection of Rous sarcoma virus. The cells have virus genome but do not normally release infectious virus. The XC plaque assay is an indirect method of detection for MuLV in cultured cells.
Rat Wistar sarcoma
Preparation Note
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
此项目有
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持