biological source
human blood
packaging
tube of 5 μg 88120801-DNA-5UG, pkg of vial of cells 88120801-1VL
growth mode
Suspension
karyotype
Modal no. = 93, Hypertetraploid
morphology
Lymphoblastoid
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Differentiation studies
Biochem/physiol Actions
Human Caucasian promyelocytic leukaemia
STR-PCR Data: Amelogenin: X
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16
CSF1PO: 14
D13S317: 8,11
D16S539: 11
D5S818: 12
D7S820: 11,12
THO1: 7
TPOX: 8,11
vWA: 16
Variant of the promyelocytic cell line HL60 isolated after treating cells with α-particle radiation and then cloned in medium containing 1.25% DMSO. Cells are capable of differentiating into neutrophils by induction with 1.75% DMSO.
Preparation Note
Maintain cultures between 3-9x100,000 cells/ml; 5% CO2; 37°C. Do not allow the culture medium to become acidic or the cell line will differentiate.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS) + 1.25% Dimethyl Sulphoxide (DMSO).
Other Notes
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法规信息
常规特殊物品
低风险生物材料
此项目有
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