生物来源
hamster ovary
包装
tube of 5 μg 89030317-DNA-5UG
pkg of vial of cells 89030317-1VL
生长模式
Adherent
核型
Not specified
形态学
Epithelial
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
生化/生理作用
Chinese hamster ovary cell line which lacks ornithine decarboxylase activity.
Hamster Chinese ovary
制备说明
EMEM (EBSS) + Ham′s F12 (1:1) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 0.1% BSA.
Split sub-confluent cultures (70-80%) 1:5 to 1:10 i.e. seeding at 5x1,000 to 1x10,000 cells/cm2 using 0.1% cold PBS and 0.25% cold trypsin. Place flasks at 4°C for 5-10 min. or until cells detach; 5% CO2; 37°C. Pre-treat culture flasks with porcine gela
其他说明
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