biological source
Rat x Rat Hybridoma
packaging
tube of 5 μg 89040603-DNA-5UG, pkg of vial of cells 89040603-1VL
ImmunoDon
DA rat spleen
Myeloma
Y3Ag1.2.3
Reactivity
Anti-L3T4 epitope B, mouse CD4
technique(s)
cell culture | mammalian: suitable
isotype
IgG2b
shipped in
dry ice
storage temp.
−196°C
Immunogen
CBA mouse spleen cells, thymocytes
Application
Not specified
Biochem/physiol Actions
Not specified
The antibody is directed against epitope B of the L3T4 antigen. This antigen is the murine equivalent of CD4. Has been used in CD4 subset depletion in vivo and flow cytometry studies. Cambridge Enterprise Reference number AB13:www.enterprise.cam.ac.uk.
Preparation Note
Iscoves Modified Dulbeccos Medium (IMDM) + 2 mM glutamine + 5% Fetal Bovine Serum (FBS). Where the cells are grown under static conditions, 0.02% EDTA, trypsin or mechanical agitation maybe required to dislodge them from the plastic.
Maintain cultures between 3-9 x 105 cells/ml; 5% CO2; 37 °C. Saturation density 9 x 105/ml
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Use of this product by commercial organizations may require a license agreement. Commercial customers will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.