biological source
Mouse x Mouse Hybridoma
clone
5, monoclonal
packaging
tube of 5 μg 89041184-DNA-5UG, pkg of vial of cells 89041184-1VL
ImmunoDon
BALB/c mouse spleen
Myeloma
NS0/1
Reactivity
Anti-CD4, rat
technique(s)
cell culture | mammalian: suitable
isotype
IgG2a
shipped in
dry ice
storage temp.
-140 to -196°C
Immunogen
T blasts prepared in mixed lymphocyte reactions with purified rat T helper cells against irradiated spleen
Biochem/physiol Actions
Mouse NS0/U x Mouse BALB/c spleen
The antibody is directed against the CD4 antigen on rat T cells and macrophages. The specificity is the same as that of W3/25, though their binding is non-competitive. OX-35 will compete with OX-36.
Preparation Note
RPMI 1640 + 2mM glutamine + 10% Foetal Bovine Serum (FBS)
Seed at 5x10,000 viable cells/ml. Subculture when cells reach 5x100,000-1x1,000,000 cells/ml (usually after 3-4 days); 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
Disclaimer
This cell line has special release conditions: Commercial organisations are required to complete the ′Cell Line Release Authorisation for Research Use in Commercial Organisations′ release conditions form.