生物来源
mouse muscle
包装
tube of 5 μg 89050906-DNA-5UG
pkg of vial of cells 89050906-1VL
生长模式
Adherent
核型
Not specified
形态学
Myoblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
生化/生理作用
Isolated from a myogenic line derived from hind limb muscle of a foetal Swiss-Webster mouse. Non-tumorigenic and highly sensitive to acetylcholine. Confluent cultures form multinucleated myotubes.
Mouse Swiss-Webster myoblast
制备说明
DMEM + 2mM Glutamine + 10% Horse Serum (HS) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:8 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C. Myotubes are formed at confluency, this is assisted by reducing both sera to 4%.
其他说明
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法规信息
新产品
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