biological source
buffalo lung
packaging
tube of 5 μg 89051704-DNA-5UG, pkg of vial of cells 89051704-1VL
growth mode
Adherent
karyotype
2n = 60
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Virus replication studies
Biochem/physiol Actions
Buffalo lung
Derived from female Buffalo yearling. The cells have a finite life span of a minimum of 30 passages and are susceptible to a range of viruses including HSV, vaccinia, PRV, BVDV, para-influenza 3, bovine enterovirus (M62/4); also IBRV and VSV (Indiana). The cell line produces both detectable BVDV antigens and infectious BVDV.
Preparation Note
McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.