Derived from a human squamous cell carcinoma (SCC) of the tongue from a 55-year-old male. SCC-4 cells have been reported to form colonies in semi-solid medium, and are not induced to differentiate by anchorage deprivation. Growth is enhanced by use of a feeder layer of 3T3 swiss cells.

Human tongue squamous carcinoma

DMEM:HAMS F12 (1:1) + 2 mM Glutamine + 10% Fetal Bovine Serum (FBS) + 0.4 μg/ml hydrocortisone

Split confluent cultures 1:3 to 1:4, i.e., seeding at 3-5 x 10,000 cells/cm² using trypsin/EDTA; 5% CO₂; 37°C

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