biological source
Human x Mouse Hybridoma hybrid
packaging
tube of 5 μg 89101606-DNA-5UG, pkg of vial of cells 89101606-1VL
growth mode
Suspension
karyotype
Not specified
morphology
Lymphoblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Fusion partner and hybridisation studies
Biochem/physiol Actions
Derived by a fusion of NS-1 - Ag4 myeloma cells with cells from a human nodular lymphoma. The cells are HPRT- and do not secrete immunoglobulin. They are used as a fusion partner for human B lymphocytes.
Mouse x Human hybrid myeloma
Preparation Note
Maintain cultures between 1 x 105 to 1 x 106 cells/ml; 5% CO2; 37°C.
RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). Genomic DNA is not available for this cell line. Use of this product by commercial organizations may require a license agreement. Commercial organizations will be contacted by cellscreening@merckgroup.com to provide further information after the order request is submitted. Any other use of this cell line is prohibited without the express written permission of UKHSA. Inquiries regarding any other authorized use of this cell line may be directed to culturecollections@ukhsa.gov.uk.