WILCL

Human Caucasian normal B cell

Normal B cell line of a patient with a lung tumour. LUDLU-23, a lung squamous cell carcinoma, is also derived from the same patient.

Maintain cultures between 3-9x100,000 cells/ml; 5% CO₂; 37°C. Cells grow as large cell aggregates; resuscitate in 8ml media with flask standing up until cells start to proliferate well.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.