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Merck
CN

NB4 1A3

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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关于此项目

UNSPSC Code:
41106514
Biological source:
mouse nerve
Relevant disease(s):
cancer
Growth mode:
Adherent
Karyotype:
Unstable
Morphology:
Neuroblast
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biological source

mouse nerve

packaging

tube of 5 μg 89121405-DNA-5UG, pkg of vial of cells 89121405-1VL

growth mode

Adherent

karyotype

Unstable

morphology

Neuroblast

products

Acetylcholinesterase, choline acetylase, tyrosine hydroxylase

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

relevant disease(s)

cancer

shipped in

dry ice

storage temp.

−196°C

Application

Tumourigenicity, enzymatic and virus studies: VSV, HSV, vaccinia virus

Biochem/physiol Actions

Cloned from the C1300 mouse neuroblastoma line. Adapted to culture by alternate in vivo and in vitro passage. Cells have characteristics of mature neurons and secrete the enzymes for the synthesis of the neurotransmitters, choline acetylase and tyrosine hydroxylase. Cells are susceptible to VSV, HSV and Vaccinia viruses.
Mouse C-1300 Neuroblastoma

Preparation Note

Ham′s F10 + 2mM Glutamine + 15% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

低风险生物材料
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历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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