Cell cycle, metabolic and mutation studies

Established from the ascitic fluid of a Novikoff hepatoma. The cells can be synchronised for cell cycle studies. N1-S1 has also been used for studies of pyrimidine metabolism, DNA synthesis and methylation and ribosomal RNA maturation.

Rat hepatoma

Maintain cultures between 4x10,000 to 1,000,000 cells/ml; 5% CO₂; 37°C. Subculture every 3-4 days by centrifugation or medium addition.

Swimm′s S-77 + 2mM Glutamine + 0.1% Pluronic F68 +10% Foetal Bovine Serum (FBS).

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