跳转至内容
Merck
CN

WI 38 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90020107, human lung (fetal), Fibroblast-like

别名:

WI38 Cells, Wistar Institute-38 Cells

登录查看公司和协议定价

选择尺寸

关于此项目

UNSPSC代码:
41106514

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

生物来源

human lung (fetal)

包装

tube of 5 μg 90020107-DNA-5UG
pkg of vial of cells 90020107-1VL

生长模式

Adherent

核型

2n = 46, diploid except at high passage number

形态学

Fibroblast-like

产品

Collagen

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

相关类别

应用

From normal female embryonic lung (expect collagen production). These cells have a very broad human virus spectra. Particularly useful for the isolation of rhinovirus. Cells from multilatered membrane when incubated for long periods with periodic pH adjustments. Used in the preparation of human virus vaccines.
Subculture: Spit at 70-80% confluency, approx. 1:3 to 1:6, seed at 2-4x104 viable cells/cm2. Trypsinize using 0.25% solution, with or without EDTA, 37 °C and 5% CO2.
Virus studies (rhinoviruses), vaccine production
WI 38 human cell line has also been used to study the cytotoxicity of acrylic based resin compounds and silica nanoparticles.

生化/生理作用

Finite lifespan. Approx. 50, +/- 10, population doublings. Normal doubling time is 24 hours.
From normal embryonic lung of female. Have very broad human virus spectra, especially useful for isolation of rhinoviruses. Cells form multilayered membrane when held for long periods at 37°C with periodic pH adjustments. Used in preparation of human virus vaccines.
Human Caucasian foetal lung
STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 11,12
D5S818: 10
D7S820: 9,11
THO1: 8,9.3
TPOX: 8
vWA: 19,20

制备说明

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Finite lifespan, 50 plus or minus 10 population doublings expected - doubling time 24h.

分析说明

Recommended culture medium: EMEM (EBSS) + 2 mM L-Glutamine + 1% Non-essential amino acids (NEAA) + 15% FBS

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

新产品
此项目有

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

  1. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  2. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  3. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  4. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持