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Merck
CN

1BR.3.N

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90020508, human skin, Fibroblast

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关于此项目

UNSPSC Code:
41106514
Biological source:
human skin
Growth mode:
Adherent
Karyotype:
Not specified
Morphology:
Fibroblast

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biological source

human skin

packaging

tube of 5 μg 90020508-DNA-5UG, pkg of vial of cells 90020508-1VL

growth mode

Adherent

karyotype

Not specified

morphology

Fibroblast

products

Not specified

receptors

Not specified

technique(s)

cell culture | mammalian: suitable

shipped in

dry ice

storage temp.

−196°C

相关类别

Application

DNA repair and cell growth studies

Biochem/physiol Actions

Derived from a normal fibroblast (1BR3 Sigma Catalogue number. 90011801) transformed with the plasmid pSV2neo. The genetic identity of 1BR3 (90011801), 1BR.3.G (90020507), 1BR.3.GN (90020508), and 1BR.3.N (90020509) has been confirmed by STR profiling at ECACC.
Human skin fibroblast, transformed
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 9,13
D13S317: 9,12
D16S539: 12,14
D5S818: 12
D7S820: 8,10
THO1: 9.3
TPOX: 11
vWA: 14,15

Preparation Note

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.

Other Notes

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

常规特殊物品
低风险生物材料
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历史批次信息供参考:

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