biological source
rat liver
packaging
tube of 5 μg 90021504-DNA-5UG, pkg of vial of cells 90021504-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Hepatocyte
products
Alpha-foetoprotein
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and oncogeny studies
Biochem/physiol Actions
Derived from the Morris hepatoma 7777 (obtained from a male Buffalo strain rat). The original tumour was induced with N-2 fluorenylphthalamic acid. Particularly interesting for oncogeny studies since the cells produce α-fetoprotein (AFP).
Rat Buffalo hepatoma
Preparation Note
Split semi-confluent cultures 1:2 to 1:6 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. NB: Cells are shed from the monolayer before confluency is achieved.
Swimm′s S-77 + 4mM Glutamine + 20% Horse Serum (HS) + 5% Foetal Bovine Serum (FBS) or EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
法规信息
低风险生物材料
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