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关于此项目
UNSPSC Code:
41106514
Biological source:
human nerve (supraorbital lymph node metastasis)
Relevant disease(s):
metastasis
Growth mode:
Adherent
Karyotype:
2n = 46, Hypodiploid to pseudodiploid, abnormalities
Morphology:
Epithelial-like
biological source
human nerve (supraorbital lymph node metastasis)
packaging
tube of 5 μg 90022302-DNA-5UG, pkg of vial of cells 90022302-1VL
growth mode
Adherent
karyotype
2n = 46, Hypodiploid to pseudodiploid, abnormalities
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
metastasis
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human neuroepithelioma, metastasis to supra-orbital area
Reported to have moderate dopamine-β-hydroxylase activity. Formaldehyde-induced fluorescence indicative of intracellular catecholamines.
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:10 to 1:20 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.