生物来源
mouse lung
包装
tube of 5 μg 90040512-DNA-5UG
pkg of vial of cells 90040512-1VL
生长模式
Adherent
核型
Unstable, range 38-256 chromosomes
形态学
Epithelial-like
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
相关疾病
cancer
运输
dry ice
储存温度
−196°C
细胞系来源
Mouse A/He lung adenoma
细胞系描述
Isolated from a urethan-induced lung adenoma of a 28 week old A/He strain mouse. The cells incorporate high levels of 14C-choline into lecithin. C-type particles have been observed in some cultures.
应用
Tumourigenicity studies
制备说明
Ham′s F12 + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 15% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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