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Merck
CN

NCTC 2071

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.

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UNSPSC代码:
41106514
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生物来源

mouse connective tissue

包装

tube of 5 μg 90050803-DNA-5UG
pkg of vial of cells 90050803-1VL

生长模式

Adherent

核型

Modal no. 63

形态学

Fibroblast

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

应用

Nutritional, immunological and toxicological studies

生化/生理作用

Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.
Mouse C3H/An connective tissue

制备说明

NCTC 135 100% + 2mM Glutamine.
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm2 by scraping off the cells and resuspending into new flasks; 5% CO2; 37°C. Freeze in 9% glycerol/91% FBS.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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分析证书(COA)

Lot/Batch Number

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