生物来源
mouse connective tissue
包装
tube of 5 μg 90050803-DNA-5UG
pkg of vial of cells 90050803-1VL
生长模式
Adherent
核型
Modal no. 63
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Nutritional, immunological and toxicological studies
生化/生理作用
Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.
Mouse C3H/An connective tissue
制备说明
NCTC 135 100% + 2mM Glutamine.
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm2 by scraping off the cells and resuspending into new flasks; 5% CO2; 37°C. Freeze in 9% glycerol/91% FBS.
其他说明
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法规信息
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