biological source
mouse connective tissue
packaging
tube of 5 μg 90050803-DNA-5UG, pkg of vial of cells 90050803-1VL
growth mode
Adherent
karyotype
Modal no. 63
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Nutritional, immunological and toxicological studies
Biochem/physiol Actions
Derivative of NCTC clone 929 and was established in chemically defined medium (NCTC 109). It was subsequently adapted to cysteine-free medium (NCTC 135). The cells have been used in nutritional, immunological and toxicological studies; nutritional, metabolic and enzyme studies; preservation and storage of viable tissue; fluid suspension culture methods and replicate culture techniques and quantitative cell culture methods.
Mouse C3H/An connective tissue
Preparation Note
NCTC 135 100% + 2mM Glutamine.
Split sub-confluent cultures (70-80%) 1:2 to 1:5 i.e. seeding at 5x10,000 to 2x100,000 cells/cm2 by scraping off the cells and resuspending into new flasks; 5% CO2; 37°C. Freeze in 9% glycerol/91% FBS.
Other Notes
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