biological source
rat unknown/unspecified
packaging
tube of 5 μg 90102526-DNA-5UG, pkg of vial of cells 90102526-1VL
growth mode
Adherent
karyotype
2n = 42
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and virus studies: Rous sarcoma virus
Biochem/physiol Actions
Derived from a Rous sarcoma virus (Schmidt-Ruppin strain) induced tumour in an inbred Amsterdam rat. The cells are highly tumorigenic in new-born rats and also chickens. There is some evidence that the cell line is latently infected with the virus.
Rat Amsterdam Schmidt-Ruppin sarcoma
Preparation Note
Medium 199 (HBSS) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.