biological source
rat unknown/unspecified
packaging
tube of 5 μg 90102526-DNA-5UG, pkg of vial of cells 90102526-1VL
growth mode
Adherent
karyotype
2n = 42
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and virus studies: Rous sarcoma virus
Biochem/physiol Actions
Derived from a Rous sarcoma virus (Schmidt-Ruppin strain) induced tumour in an inbred Amsterdam rat. The cells are highly tumorigenic in new-born rats and also chickens. There is some evidence that the cell line is latently infected with the virus.
Rat Amsterdam Schmidt-Ruppin sarcoma
Preparation Note
Medium 199 (HBSS) + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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