Cri du Chat

Chromosome studies

Derived from the skin of an adult female Caucasian with Cri du Chat syndrome. The cells have a deletion in chromosome 5 and have a finite lifespan; up to 35 population doublings expected.

Human Caucasian skin deletion in chromosome 5

McCoy′s 5a + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 i.e. seeding at 5x10,000 cells/cm² using 0.25% trypsin; 5% CO₂; 37°C. Cells should not be centrifuged.

Cultures from HPA Culture Collections and supplied by Sigma are for research purposes only. Enquiries regarding the commercial use of a cell line are referred to the depositor of the cell line. Some cell lines have additional special release conditions such as the requirement for a material transfer agreement to be completed by the potential recipient prior to the supply of the cell line. Please view the Terms & Conditions of Supply for more information.