biological source
mouse breast
packaging
tube of 5 μg 90110502-DNA-5UG, pkg of vial of cells 90110502-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Epithelial
products
A-type and C-type virus particles, plasminogen activator (tPA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Non malignant mammary cell studies.
Biochem/physiol Actions
Derived from pre-malignant mammary tissue of a BALB/c mouse. Produce significant amounts of C-type and A-type virus particles and low amounts of plasminogen activator.
Mouse BALB/c mammary alveolar nodules, pre-neoplastic
Preparation Note
DMEM + 2mM Glutamine + 10μg/ml Insulin + 10% Foetal Bovine Serum (FBS).
Split semi-confluent cultures 1:2 to 1:4 i.e. seeding at 3-5x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.