生物来源
human skin
包装
tube of 5 μg 90110503-DNA-5UG
pkg of vial of cells 90110503-1VL
生长模式
Adherent
核型
Modal no. 46
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Tumourigenicity studies
生化/生理作用
Derived from a 13 year old Caucasian male with Gardner′s syndrome, an autosomal dominant condition which predisposes to carcinoma and multiple polyps of the colon. The modal chromosome number was 46, occurring in 60% of cells.
Human Caucasian skin fibroblast Gardner′s syndrome
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11
D13S317: 11,12
D16S539: 12,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 14,17
CSF1PO: 11
D13S317: 11,12
D16S539: 12,13
D5S818: 11,12
D7S820: 9,10
THO1: 7,8
TPOX: 8,11
vWA: 14,17
制备说明
EMEM (HBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 20% Foetal Bovine Serum (FBS).
Split semi-confluent cultures 1:2 i.e. seeding at 4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C. Monolayer should form in a 25cm2 flask in 3-4 days.
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