biological source
human placenta
packaging
tube of 5 μg 90110508-DNA-5UG, pkg of vial of cells 90110508-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Human chorionic gonadotropin (hCG)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Human placenta (post crisis); SV40 transformed
STR-PCR Data: Amelogenin: X
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,17
CSF1PO: 10,12
D13S317: 11
D16S539: 9,12
D5S818: 11,13
D7S820: 10
THO1: 9,9.3
TPOX: 8,11
vWA: 16,17
The cell line 3A sub E was derived from 3A(tPA-30-1)after the original line started to senesce and went through crisis. 3A subE has an unlimited lifespan and expresses human chorionic gonadotropin (hCG)
Preparation Note
Aspirate medium and add 0.25% trypsin/EDTA; incubate at room temperature until cells detach. Dilute with fresh medium and aliquot into new flasks at a split ratio 1:2. Renew fluid 1-2 times per week. Incubate culture at 37°C, 5% CO2 for initial 24 hour
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS)
Other Notes
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法规信息
低风险生物材料
常规特殊物品
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