biological source
mouse unknown/unspecified
packaging
tube of 5 μg 90110707-DNA-5UG, pkg of vial of cells 90110707-1VL
growth mode
Suspension
karyotype
Modal no. 43, 81-85 hyperdiploid
morphology
Lymphoblast
products
Immunoglobulin G (IgG1)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Mouse BALB/c plasmacytoma, IgG secreting
The original plasma cell tumour was induced by intraperitoneal injections of mineral oil into highly inbred BALB/cJ mice. The line secretes IgG1, is hyperdiploid and produces a balanced synthesis of monomeric 4-chain gamma F/immunoglobulin.
Preparation Note
L15 + 2mM Glutamine + 20% Foetal Bovine Serum (FBS).
Maintain cultures between 3-9x100,000 cells/ml; 37°C. The cells have a maximum density of 1-2x1,000,000 cells/ml. CO2 is not required.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
法规信息
动物来源培养基
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