biological source
rat bone
packaging
tube of 5 μg 90111314-DNA-5UG, pkg of vial of cells 90111314-1VL
growth mode
Adherent
karyotype
Not specified
morphology
Not specified
products
Not specified
receptors
Cytosol receptors for the bone resorbing steroid hormone 1,25 -(OH)2 D3.
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity and hormone studies
Biochem/physiol Actions
A cloned derivative of a transplantable rat osteogenic sarcoma. The cells retain many of the characteristics of the tumour including responsiveness to parathyroid hormone (PTH) and prostaglandins.
Rat osteosarcoma
Preparation Note
DMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:10 i.e. seeding at 1-3x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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