biological source
human cervix
packaging
tube of 5 μg 90111910-DNA-5UG, pkg of vial of cells 90111910-1VL
growth mode
Adherent
karyotype
Modal no. 71
morphology
Epithelial-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Virus, tumourigenicity and chromosome studies
Biochem/physiol Actions
Human cervix carcinoma
Originally derived from normal amniotic tissue. Have been used and studied extensively including virus susceptibility, tumorigenicity and morphology. Contains HeLa marker chromosomes and exhibits type A G6PD. This cell line was found to be indistinguishable from HeLa by STR PCR DNA profiling. Therefore, the cell line should be considered as derived from HeLa. The cells are capable of growth in a variety of media supplemented with sera from several species. Ethnicity: Black.
Preparation Note
EMEM + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:5 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin; 5% CO2; 37°C.
Other Notes
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