biological source
rat unknown/unspecified
packaging
tube of 5 μg 90111913-DNA-5UG, pkg of vial of cells 90111913-1VL
growth mode
Adherent
karyotype
2n = 42, hyperdiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Metabolic and cloning studies
Biochem/physiol Actions
Rat sarcoma, asparagine requiring
Used in studies relating to amino acid requirements and L-asparagine metabolism. Cloning studies show that variants not requiring L-asp are present in 100 per 1,000,000 cells Cloned populations which do not produce variants immediately do so after 6-8 further passages.
Preparation Note
McCoy′s 5a + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Renew medium daily where possible. Subculture by placing culture in cold room (4°C) for 60 minutes, shake flask to suspend cells, centrifuge, resuspend in fresh medium and into fresh flasks. Split sub-confluent cultures (70-80%) 1:2 to 1:10; 5% CO2; 37°C.
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.
法规信息
动物来源培养基
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