biological source
rat unknown/unspecified
packaging
tube of 5 μg 90111913-DNA-5UG, pkg of vial of cells 90111913-1VL
growth mode
Adherent
karyotype
2n = 42, hyperdiploid
morphology
Fibroblast
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Metabolic and cloning studies
Biochem/physiol Actions
Rat sarcoma, asparagine requiring
Used in studies relating to amino acid requirements and L-asparagine metabolism. Cloning studies show that variants not requiring L-asp are present in 100 per 1,000,000 cells Cloned populations which do not produce variants immediately do so after 6-8 further passages.
Preparation Note
McCoy′s 5a + 2mM Glutamine + 5% Foetal Bovine Serum (FBS).
Renew medium daily where possible. Subculture by placing culture in cold room (4°C) for 60 minutes, shake flask to suspend cells, centrifuge, resuspend in fresh medium and into fresh flasks. Split sub-confluent cultures (70-80%) 1:2 to 1:10; 5% CO2; 37°C.
Other Notes
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法规信息
低风险生物材料
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