WIL2 NS

Carcinogenesis studies

A non secreting B lymphocyte variant of WIL-2, which was originally isolated from the spleen of a Caucasian male with hereditary spherocytic anaemia.

Human Caucasian B lymphocyte

STR-PCR Data: Amelogenin: X,Y
CSF1PO: 11,12
D13S317: 11
D16S539: 11,12
D5S818: 12,13
D7S820: 9,12
THO1: 8,9.3
TPOX: 8,11
vWA: 17,20

Maintain cultures between 2x100,000 to 1,000,000 cells/ml; 5% CO₂; 37°C.

RPMI 1640 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.