biological source
human lung
packaging
tube of 5 μg 90112704-DNA-5UG, pkg of vial of cells 90112704-1VL
growth mode
Adherent
karyotype
2n = 46
morphology
Fibroblast-like
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Established from lung tissue of a 71 year old male with pancreatic carcinoma and neoplastic invasion of the spleen and stomach and a mild case of emphysema. The cells can undergo up to 20 population doublings. Ethnicity: Black.
Human lung
STR-PCR Data: Amelogenin: X,Y
CSF1PO: 12
D13S317: 10,14
D16S539: 9,11
D5S818: 10,12
D7S820: 10
THO1: 7,9.3
TPOX: 9,11
vWA: 14,17
CSF1PO: 12
D13S317: 10,14
D16S539: 9,11
D5S818: 10,12
D7S820: 10
THO1: 7,9.3
TPOX: 9,11
vWA: 14,17
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:3 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.