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PC-3 Cell Line human

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 90112714, human prostate, Epithelial

别名:

PC.3 Cells, PC3 Cells

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UNSPSC代码:
41106514
eCl@ss:
32190102

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生物来源

human prostate

包装

tube of 5 μg 90112714-DNA-5UG
pkg of vial of cells 90112714-1VL

生长模式

Adherent

核型

2n = 46, triploid, modal no. 62

形态学

Epithelial

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

相关疾病

cancer

运输

dry ice

储存温度

−196°C

应用

PC-3 cell line has been used to study the antiproliferative effects of F. mughlea Pesmen on cancer cells. It has also been used to study the effects of palmitoylcarnitine (palcar) on the gene expression and pro-inflammatory pathways in prostate cancer.
Tumourigenicity studies

生化/生理作用

Established from a grade 4 prostatic adenocarcinoma from a 62 year old male Caucasian. The cells grow in agar and produce tumours in nude mice. Exhibit low acid phosphatase and testosterone-5-α reductase activity. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Human Caucasian prostate adenocarcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 11
D13S317: 11
D16S539: 11
D5S818: 13
D7S820: 8,11
THO1: 6,7
TPOX: 8,9
vWA: 17

制备说明

Coons Modified Ham’s F12 + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).OR Kaign’s modified Ham’s F12 + 45mg/L ascorbic acid + 18 mg/L Inositol + 2mM Glutamine + 7% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:2 to 1:6 i.e. seeding at 2-5x10,000 cells/cm2 using 0.25% trypsin/EDTA; 5% CO2; 37°C. Medium change every 5 days. The initial subculture interval after cells are thawed may be longer than 7-9 days.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

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