biological source
human mouth (embryonic)
packaging
tube of 5 μg 90120505-DNA-5UG, pkg of vial of cells 90120505-1VL
growth mode
Adherent
karyotype
2n = 46, diploid
morphology
Epithelial
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Application
Proliferation studies
Biochem/physiol Actions
Established from embryonic palatal shelves. The cells are very sensitive to the stimulating effect of EGF with respect to proliferation, incorporation of thymidine and ornithine decarboxylase.
Human embryonic palatal mesenchyme
Preparation Note
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
Other Notes
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法规信息
常规特殊物品
低风险生物材料
此项目有
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