产品名称
L35, NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA.
biological source
Porcine blood
manufacturer/tradename
ECACC
packaging
tube of 5 μg 91012319-DNA-5UG, pkg of vial of cells 91012319-1VL
growth mode
Suspension
karyotype
Modal no. 36.5
morphology
Not specified
products
Not specified
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
shipped in
dry ice
storage temp.
−196°C
Biochem/physiol Actions
Not specified
One of a series of cell lines established from mononuclear cells of a boar (Sus scrofa domestica L) which was injected with Shimozuma cells producing porcine retrovirus (Tsukuba-1). The cells express MSA4. The cells contain retrovirus particles and are positive in RT assay.
Porcine peripheral blood T cell
Preparation Note
Maintain cultures between 100,000 to 1,000,000 cells/ml; 5% CO2; 37 °C. Cells grow as small clumps.
RPMI 1640 + 2 mM Glutamine + 1 mM Sodium Pyruvate (NaP) + 0.05 mM 2-Mercaptoethanol (2ME) + 10% Fetal Bovine Serum (FBS).
Other Notes
Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.
This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.