生物来源
bovine adrenal gland (cortex)
包装
tube of 5 μg 91021201-DNA-5UG
pkg of vial of cells 91021201-1VL
生长模式
Adherent
核型
Not specified
形态学
Fibroblast
产品
Not specified
受体
Not specified
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
应用
Hormone studies
生化/生理作用
Bovine adrenal cortex, normal
Derived from the fasciculata-reticularis zone of the adrenal cortex taken from three normal adult cows. FGF stimulates cell division. Cells are responsive to ACTH and other steroidogenic agents. Finite lifespan; 60-70 population doublings expected. Studies have indicated that this cell line produces both detectable BVDV antigens and infectious BVDV.
制备说明
Ham′s F12 with Fibroblast Growth Factor (FGF) (crude, 50ng/ml or pure, 500 pg/ml) + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
其他说明
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