生物来源
opossum kidney
包装
tube of 5 μg 91021202-DNA-5UG
pkg of vial of cells 91021202-1VL
生长模式
Adherent
核型
Not specified
形态学
Not specified
产品
Not specified
受体
Insulin, serotonin, alpha 2-adrenergic parathy. Hormone
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
American Opossum, kidney
细胞系描述
Derived from proximal tubule of a kidney taken from an adult female opossum. Cells retain proximal tubule function. Possesses the following receptors: α 2 - adrenergic, insulin, serotonin, parathyroid hormone and atrial natriuretic factor.
应用
Kidney function studies
培养基
EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:2 to 1:4 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37°C.
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