Detroit 539

Chromosome studies

Derived from a female caucasian infant with Down Syndrome. Karyotypic analysis shows 90% of the cell population possesses 47 chromosomes with 5 chromosomes belonging to G group. These findings persist essentially unchanged throughout at least 30 serial subcultures.

Human Caucasian skin Down syndrome

STR-PCR Data: Amelogenin: X
CSF1PO: 9,10
D13S317: 11,13
D16S539: 13
D5S818: 11,13
D7S820: 9,11
THO1: 8,9
TPOX: 8,10
vWA: 16,18

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 1mM Sodium Pyruvate (NaP) + 0.1% Lactalbumin hydrolysate (LH) + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:4 i.e. seeding at 3-5x10,000 cells/cm² using 0.25% trypsin or trypsin/EDTA; 5% CO₂; 37°C. A further 15-20 passages expected.

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This cell line is part of the European Collection of Authenticated Cell Cultures (ECACC), an international repository managed by the United Kingdom Health Security Agency (UKHSA). No licensing agreement is required when either this cell line or the DNA extracted from it are used for internal research purposes only. Any other use of these products is prohibited without the express written permission of UKHSA. Inquiries regarding authorized use of this cell line or its genomic DNA may be directed to culturecollections@ukhsa.gov.uk.