biological source
human colon
packaging
tube of 5 μg 91031104-DNA-5UG, pkg of vial of cells 91031104-1VL
growth mode
Adherent
karyotype
2n = 46, hypodiploid
morphology
Epithelial
products
Carcinoembryonic antigen (CEA)
receptors
Not specified
technique(s)
cell culture | mammalian: suitable
relevant disease(s)
cancer
shipped in
dry ice
storage temp.
−196°C
Application
Tumourigenicity studies
Biochem/physiol Actions
Established from a Grade IV adenocarcinoma of the rectum of a 53 year old Caucasian male. The cells produce CEA and electron microscopy reveals brush borders. The Y chromosome could not be detected in this cell line by short tandem repeat (STR)-PCR analysis when tested at ECACC. It is a known phenomenon that due to the increased genetic instability of cancer cell lines the Y chromosome can be rearranged or lost resulting in lack of detection. The cell line is identical to the source provided by the depositor based on the STR-PCR analysis.
Human Caucasian rectum adenocarcinoma
STR-PCR Data: Amelogenin: X
CSF1PO: 10
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,9
vWA: 15,16
CSF1PO: 10
D13S317: 13
D16S539: 12
D5S818: 12
D7S820: 9,12
THO1: 9.3
TPOX: 8,9
vWA: 15,16
Preparation Note
L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-4x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; No CO2; 37°C. After thawing the 1st subculture interval may be 14-18 days. Medium change after 4 days.
Other Notes
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法规信息
低风险生物材料
常规特殊物品
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