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CHSE-214 Cell Line from salmon

NOTE: Both the cell line and DNA from the cell line may be available for this product. Please choose -1VL or VIAL for cells, or -DNA-5UG for DNA, 91041114, fish embryo, Not specified

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UNSPSC代码:
41106514

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生物来源

fish embryo

包装

tube of 5 μg 91041114-DNA-5UG
pkg of vial of cells 91041114-1VL

生长模式

Adherent

核型

Not specified

形态学

Not specified

产品

Not specified

受体

Not specified

技术

cell culture | mammalian: suitable

运输

dry ice

储存温度

−196°C

应用

CHSE-214 cell line has been used for the growth and titration of infectious pancreatic necrosis virus (IPNV) from samples. It has also been used to carry out cell infection assays to determine the effect of tropodithietic acid (TDA)-exposed isolates.

生化/生理作用

Derived from a Chinook salmon (Oncorhynchus tshawytscha) embryo, susceptible to a wide range of fish viruses and in many instances replicate high titres.
Salmon embryo

制备说明

EMEM (EBSS) + 2mM Glutamine + 1% Non Essential Amino Acids (NEAA) + 10% Foetal Bovine Serum (FBS).
Split sub-confluent cultures (70-80%) 1:3 to 1:6; i.e seeding at 1-4 x 10,000 cells per cm using 0.25% trypsin or trypsin/EDTA, cells detach after 5-10 minutes at room temperature. Grow cells in dark (e.g. wrapped in foil); 5% CO2; 21°C. Freeze cells in 5% DMSO and 95% foetal bovine serum (FBS). Fish cell lines detach easily during transit if the culture is too young. For resuscitation seed flasks at 4-5 x 10,000 cells per cm and then split cells 1:3 to 1:4 and culture for at least one week with 1-2 media changes before shipping.

其他说明

Additional freight & handling charges may be applicable for Asia-Pacific shipments. Please check with your local Customer Service representative for more information.

法规信息

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分析证书(COA)

Lot/Batch Number

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    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

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