生物来源
mouse testis
包装
tube of 5 μg 91060526-DNA-5UG
pkg of vial of cells 91060526-1VL
生长模式
Adherent
核型
Not specified
形态学
Not specified
产品
Prostaglandin F2a
受体
Epidermal growth factor (EGF), Androgen, Oestrogen, Progesterone
技术
cell culture | mammalian: suitable
运输
dry ice
储存温度
−196°C
相关类别
细胞系来源
Mouse BALB/c testis, Leydig cell
细胞系描述
Originated from primary cultures of Leydig cell enriched preparations from normal testes of 11-13 day old BALB/c nu/+ mice. Characteristics similar to primary cultures of Leydig cells. Responds to leuteinising hormone but not FSH. In the presence of LH the cells are capable of metabolising cholesterol. These cells are non-tumourigenic.
应用
Hormone studies
培养基
Ham′s F12 + DMEM (1:1) + 2mM Glutamine + 5% Horse Serum (HS) + 2.5% Foetal Bovine Serum (FBS).
传代培养常规
Split sub-confluent cultures (70-80%) 1:5 to 1:200 i.e. seeding at 1x10,000 cells/cm2 using 0.25% trypsin or trypsin/EDTA; 5% CO2; 37²C.
其他说明
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