SW 626

Although originally thought to be of ovarian origin, a recent report has suggested that the SW 686 cell line might have been derived from an ovarian metastasis of a primary adenocarcinoma of the colon Furlong, et al., 1999. The SW 626 cell line was initiated by A. Leibovitz in January 1974 at the Scott and White Clinic, Temple, Texas from a surgical specimen from a cystadenocarcinoma of the ovary in a 46 year old female Caucasian. The histopathology of the specimen was determined to be grade III adenocarcinoma.

Human Caucasian ovarian metastasis of a primary colon adenocarcinoma

L15 + 2mM Glutamine + 10% Foetal Bovine Serum (FBS).

Split sub-confluent cultures (70-80%) 1:3 to 1:6 i.e. seeding at 2-3x10,000 cells/cm² using 0.25% trypsin/EDTA; CO₂ is not needed. Medium change after 4-5 days. 37°C.

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